Evolution of Eucaryotic Cells

Detection of DNA methylation in eucaryotic cells.

The methods of molecular biology allow for analyzing the methylation pattern in the whole genome and in particular genes. We differentiate methylated sequences from unmethylated ones by means of cutting the genomic template with methylation-sensitive restriction enzymes or by sodium bisulfite DNA modification. Chemical modification precedes most quantitative and qualitative PCR techniques: MS-P...

Steroid hormone action in eucaryotic cells.

Effects of beta-lactam antibiotics on proliferating eucaryotic cells.

Most fungal toxins interact specifically with well-defined target structures, thereby reaching very high selectivity for the cell type attacked (17). The susceptibility of bacteria to beta-lactam antibiotics, as an example, is due to a number of penicillin-binding proteins on the bacterial plasma membrane. The eucaryotic cell lacks such target structures. Its insusceptibility to beta-lactams se...

Rapid method of total RNA mini-preparation from eucaryotic cells.

There have been described a wide variety of methods for the preparation of total RNA from eucaryotic cells (1, 2). These methods are mainly designed for the isolation of RNA from relatively large amounts of cells. In a situation of screening a large number of cell clones, minimized cell cultivation effort and preparation time for total RNA isolation will be of great advantage. We developed a me...

Binding of pertussis toxin to eucaryotic cells and glycoproteins.

The binding of pertussis toxin and its subunits to cell surface receptors and purified glycoproteins was examined. The interaction of pertussis toxin with components of two variant Chinese hamster ovary (CHO) cell lines was studied. These cell lines are deficient in either sialic acid residues (LEC 2) or sialic acid and galactose residues (LEC 8) on cell surface macromolecules. The binding of p...